Tag: CRISPR technology

The Future of Medicine: CRISPR Technology vs HIV Virus

HIV virus has been a global problem for decades now, and even though scientists did make progress in battling this illness, these is no definite solution (so far). So it is surprising that the answer might lay in CRISPR technology, primarily developed for genetically modifying crops and plants. CRISPR gene editing method allows scientists to identify and locate the DNA sequence they want to modify and basically improve it by a copy/paste method. So the end result is an edited organism that does not contain any outside DNA.

CRISPR vs HIV

A team of scientists working at the Temple University located in Philadelphia decided to take a closer look at CRISPR/Cas 9 technology and find the way to use it in order to remove HIV-1 genome from human CD4+T cells. These cells are in charge of immune system. Their attempt was successful and HIV-1 genome disappeared completely from the infected cells. This finding might be a watershed moment in our history because HIV-1 is the main source of HIV infections.

Further analysis of the few treated individuals revealed that HIV-1 replication has slowed down and that their general health has improved. These astonishing results are a proof that immune system can repair itself and that CD4+T cells will not be damaged by this brand new treatment. As a matter of fact, the small number of patients who were involved in this study showed a huge progress in a very short period of time.

As we have previously mentioned, the host genome was not affected in any way after this treatment. Their DNA remained unchanged after the successful removal of HIV-1 from the immune cells. There are even indications that the treated cells are almost immune to a new HIV-1 infection, but this claim has to be explored furthermore. However, one thing is certain – the viral load of the treated patients decreased significantly. The research team from Temple University still have plenty of work ahead of them for sure.

The cure for HIV-1?

This trial was not large and it included only a handful of individuals that are infected with HIV-1 virus. It is certainly a huge step forward because this finding might lead us straight to the cure. Various drug therapies are available for the infected patients and they allow them to live a normal life for years to come. However, the virus is still present in their system and it might affect them in the long run if they are not careful. Any step away from the prescribed therapy might bring the virus back to the surface. So yes, once the virus gets into human system, there is no going back.

What makes CRISPR/Cas 9 technology so important is the fact that it can be used to target specific areas of a cell, and modify the parts that need repair. The fact that the technology developed for genetically modifying crops can be used to save thousands and thousands of lives and it might be the solution we have all been waiting for decades is very exciting. Temple University will continue their research and we are all excited to see the results.

Genetically Modified Mosquitos to Battle Zika Virus

Zika virus was all over the headlines this year and it became a real danger to anyone visiting South America. It is spread by mosquito bites and scientists are having a really hard time containing the virus in one place. Since the cure is nowhere in sight, researchers are hoping to find an alternative solution to this problem.

As a matter of fact, two scientists from Virginia Tech decided to tackle the issue head on and try to fight it from within by applying the latest technologies that involve genetically modifying mosquitos. Zuch Adolman and Zhijen Thu started developing a new method of fighting the virus by reducing the number of female mosquitos in one place because they are the ones who are spreading the virus.

So how do they plan to lower the number of female mosquitos? They came up with a new way to convert the female mosquitos to males by genetically modifying their code. The same scientists managed to make alterations to female mosquitos who were spreading malaria and sterilize them by using CRISPR-Cas9 gene modifying technology. They plan to do something similar with mosquitos who are the carriers of the Zika virus.

They have already identified the gene that is responsible for determining the gender of a mosquito and by applying the so called Nix gene to female mosquitos, they were successful in turning them into male mosquitos. That is particularly useful because female mosquitos are the ones who suck blood and transmit the virus via bite. By reducing the number of female mosquitos, a certain area will be less populated with these bugs and the possibility of getting bitten by a mosquito that is a Zika virus carrier will be significantly reduced.

If these two scientists manage to perfect this method of controlling the mosquito population, we will be able to battle various illnesses that are capable of spreading by mosquito bites. It is an excellent way to keep the mosquito population in check without wreaking havoc on the surrounding ecosystem.

CRISPR technology differs from the standard GMOs due to the fact it doesn’t introduce any foreign genes to the organisms that it is modifying. It became pretty well known in the scientific circles because it was successfully applied to mushrooms in order to prevent them from changing color from white to brown, prolonging their shelf life. So CRISPR improves and strengthens the already existing genes within a given species.

Surely, the team from Virginia Tech will need more time to test their findings in a controlled environment so the chances are we won’t be seeing the results of their hard work for the next couple of months. CRISPR gene modifying technology is still a new concept that requires more trials, but they already have a solid foundation because the same team of scientists worked on a similar project. If they do succeed, this method will reduce Zika virus carriers and save thousands of lives all around the globe. Mosquito borne diseases are really hard to fight so hopefully we’ll finally have a solution that will eliminate the threat.